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cell lines human embryonic kidney 293t hek293t american type culture collection  (ATCC)


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    Structured Review

    ATCC cell lines human embryonic kidney 293t hek293t american type culture collection
    Cell Lines Human Embryonic Kidney 293t Hek293t American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+human+embryonic+kidney+293t+american+type+culture+collection/293T/pm41709459-589-86-93
    Average 99 stars, based on 38021 article reviews
    cell lines human embryonic kidney 293t hek293t american type culture collection - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: Divergent viral phosphodiesterases for immune signaling evasion.
    Article Snippet: HEK293T cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Corning 10-013-CV) containing 4.5 g/L glucose, L-glutamine and sodium pyruvate and supplemented with 10% Fetal Bovine Serum (Avantor 97068-085) at 37 ◦ C and 5% CO 2 . .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphodiesterase from Burkholderia phage BCSR5 in the closed lid conformation This paper PDB: 9Q2G Structures and sequences of 2H PDEs Zenodo 10.5281/zenodo.16709871 INfrastructure for a PHAge REference Database (INPHARED) Cook et al. 30 N/A Megaphages from European Nucleotide Archive (ENA) Al-Shayeb et al. 31 Project #PRJEB35371 Experimental models: Cell lines Human embryonic kidney 293T American Type Culture Collection Cat# RL-3216 Recombinant DNA pMSCV-hygro-STING R232 Addgene Cat# 102608 IFN-Beta_pGL3 Addgene Cat# 102597 pRL-TK Vector Promega Cat# PAE2241 Custom pET-based vector (backbone used for expression of all PDEs) Addgene Cat # 246070 Software and algorithms Colabfold Mirdita et al. 32 https://github.com/sokrypton/ColabFold vpSAT Nomburg et al. 7 https://github.com/jnoms/vpSAT Original code for 2H PDE analysis This work 10.5281/zenodo.16709871 MMseqs2 Steinegger et al. 33 https://github.com/soedinglab/MMseqs2 DaliLite Holm et al. 12 http://ekhidna2.biocenter.helsinki.fi/dali/ Foldmason version 1.763a428 Gilchrist et al. 15 https://github.com/steineggerlab/foldmason CATH Waman et al. 34 cath-dataset-nonredundant-S20.pdb.tgz CCP4i2 Potterton et al. 35 https://www.ccp4.ac.uk/ download/#os=macos Phenix Adams et al. 36 https://www.phenix-online.org Xcalibur version 2.0.7 Thermo Fisher Cat# OPTON-30965 msconvert Galaxy community 37 Galaxy platform MZmine 3.9.0 Schmid et al. 38 https://mzmine.github.io/mzmine_ documentation/index.html Gel Analyzer 19.0 Web www.gelanalyzer.com Cell Host & Microbe 33, 1–9.e1–e6, December 10, 2025 e2 ..

    Plasmid Preparation:

    Article Title: Divergent viral phosphodiesterases for immune signaling evasion.
    Article Snippet: HEK293T cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Corning 10-013-CV) containing 4.5 g/L glucose, L-glutamine and sodium pyruvate and supplemented with 10% Fetal Bovine Serum (Avantor 97068-085) at 37 ◦ C and 5% CO 2 . .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphodiesterase from Burkholderia phage BCSR5 in the closed lid conformation This paper PDB: 9Q2G Structures and sequences of 2H PDEs Zenodo 10.5281/zenodo.16709871 INfrastructure for a PHAge REference Database (INPHARED) Cook et al. 30 N/A Megaphages from European Nucleotide Archive (ENA) Al-Shayeb et al. 31 Project #PRJEB35371 Experimental models: Cell lines Human embryonic kidney 293T American Type Culture Collection Cat# RL-3216 Recombinant DNA pMSCV-hygro-STING R232 Addgene Cat# 102608 IFN-Beta_pGL3 Addgene Cat# 102597 pRL-TK Vector Promega Cat# PAE2241 Custom pET-based vector (backbone used for expression of all PDEs) Addgene Cat # 246070 Software and algorithms Colabfold Mirdita et al. 32 https://github.com/sokrypton/ColabFold vpSAT Nomburg et al. 7 https://github.com/jnoms/vpSAT Original code for 2H PDE analysis This work 10.5281/zenodo.16709871 MMseqs2 Steinegger et al. 33 https://github.com/soedinglab/MMseqs2 DaliLite Holm et al. 12 http://ekhidna2.biocenter.helsinki.fi/dali/ Foldmason version 1.763a428 Gilchrist et al. 15 https://github.com/steineggerlab/foldmason CATH Waman et al. 34 cath-dataset-nonredundant-S20.pdb.tgz CCP4i2 Potterton et al. 35 https://www.ccp4.ac.uk/ download/#os=macos Phenix Adams et al. 36 https://www.phenix-online.org Xcalibur version 2.0.7 Thermo Fisher Cat# OPTON-30965 msconvert Galaxy community 37 Galaxy platform MZmine 3.9.0 Schmid et al. 38 https://mzmine.github.io/mzmine_ documentation/index.html Gel Analyzer 19.0 Web www.gelanalyzer.com Cell Host & Microbe 33, 1–9.e1–e6, December 10, 2025 e2 ..

    Expressing:

    Article Title: Divergent viral phosphodiesterases for immune signaling evasion.
    Article Snippet: HEK293T cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Corning 10-013-CV) containing 4.5 g/L glucose, L-glutamine and sodium pyruvate and supplemented with 10% Fetal Bovine Serum (Avantor 97068-085) at 37 ◦ C and 5% CO 2 . .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphodiesterase from Burkholderia phage BCSR5 in the closed lid conformation This paper PDB: 9Q2G Structures and sequences of 2H PDEs Zenodo 10.5281/zenodo.16709871 INfrastructure for a PHAge REference Database (INPHARED) Cook et al. 30 N/A Megaphages from European Nucleotide Archive (ENA) Al-Shayeb et al. 31 Project #PRJEB35371 Experimental models: Cell lines Human embryonic kidney 293T American Type Culture Collection Cat# RL-3216 Recombinant DNA pMSCV-hygro-STING R232 Addgene Cat# 102608 IFN-Beta_pGL3 Addgene Cat# 102597 pRL-TK Vector Promega Cat# PAE2241 Custom pET-based vector (backbone used for expression of all PDEs) Addgene Cat # 246070 Software and algorithms Colabfold Mirdita et al. 32 https://github.com/sokrypton/ColabFold vpSAT Nomburg et al. 7 https://github.com/jnoms/vpSAT Original code for 2H PDE analysis This work 10.5281/zenodo.16709871 MMseqs2 Steinegger et al. 33 https://github.com/soedinglab/MMseqs2 DaliLite Holm et al. 12 http://ekhidna2.biocenter.helsinki.fi/dali/ Foldmason version 1.763a428 Gilchrist et al. 15 https://github.com/steineggerlab/foldmason CATH Waman et al. 34 cath-dataset-nonredundant-S20.pdb.tgz CCP4i2 Potterton et al. 35 https://www.ccp4.ac.uk/ download/#os=macos Phenix Adams et al. 36 https://www.phenix-online.org Xcalibur version 2.0.7 Thermo Fisher Cat# OPTON-30965 msconvert Galaxy community 37 Galaxy platform MZmine 3.9.0 Schmid et al. 38 https://mzmine.github.io/mzmine_ documentation/index.html Gel Analyzer 19.0 Web www.gelanalyzer.com Cell Host & Microbe 33, 1–9.e1–e6, December 10, 2025 e2 ..

    Software:

    Article Title: Divergent viral phosphodiesterases for immune signaling evasion.
    Article Snippet: HEK293T cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Corning 10-013-CV) containing 4.5 g/L glucose, L-glutamine and sodium pyruvate and supplemented with 10% Fetal Bovine Serum (Avantor 97068-085) at 37 ◦ C and 5% CO 2 . .. REAGENT or RESOURCE SOURCE IDENTIFIER Phosphodiesterase from Burkholderia phage BCSR5 in the closed lid conformation This paper PDB: 9Q2G Structures and sequences of 2H PDEs Zenodo 10.5281/zenodo.16709871 INfrastructure for a PHAge REference Database (INPHARED) Cook et al. 30 N/A Megaphages from European Nucleotide Archive (ENA) Al-Shayeb et al. 31 Project #PRJEB35371 Experimental models: Cell lines Human embryonic kidney 293T American Type Culture Collection Cat# RL-3216 Recombinant DNA pMSCV-hygro-STING R232 Addgene Cat# 102608 IFN-Beta_pGL3 Addgene Cat# 102597 pRL-TK Vector Promega Cat# PAE2241 Custom pET-based vector (backbone used for expression of all PDEs) Addgene Cat # 246070 Software and algorithms Colabfold Mirdita et al. 32 https://github.com/sokrypton/ColabFold vpSAT Nomburg et al. 7 https://github.com/jnoms/vpSAT Original code for 2H PDE analysis This work 10.5281/zenodo.16709871 MMseqs2 Steinegger et al. 33 https://github.com/soedinglab/MMseqs2 DaliLite Holm et al. 12 http://ekhidna2.biocenter.helsinki.fi/dali/ Foldmason version 1.763a428 Gilchrist et al. 15 https://github.com/steineggerlab/foldmason CATH Waman et al. 34 cath-dataset-nonredundant-S20.pdb.tgz CCP4i2 Potterton et al. 35 https://www.ccp4.ac.uk/ download/#os=macos Phenix Adams et al. 36 https://www.phenix-online.org Xcalibur version 2.0.7 Thermo Fisher Cat# OPTON-30965 msconvert Galaxy community 37 Galaxy platform MZmine 3.9.0 Schmid et al. 38 https://mzmine.github.io/mzmine_ documentation/index.html Gel Analyzer 19.0 Web www.gelanalyzer.com Cell Host & Microbe 33, 1–9.e1–e6, December 10, 2025 e2 ..



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